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KMID : 0370220110550010064
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Yakhak Hoeji
2011 Volume.55 No. 1 p.64 ~ p.68
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Simultaneous Analysis of three Marker Components in Hwangryunhaedok-tang by HPLC-DAD
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Yang Hye-Jin
Weon Jin-Bae
Ma Jin-Yeul
Ma Choong-Je
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Abstract
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In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, C₁? column (5㎛ , 4.6 mm250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of 30℃. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity (R² > 0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 ㎍/㎖ and 0.15 to 0.53 ㎍/㎖, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.
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KEYWORD
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Hwangryunhaedok-tang, HPLC-DAD, simultaneous determination, validation
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